Pittsburgh drug Discovery Institute // Drug Repurposing

Pittsburgh drug Discovery Institute

Discovery Technology

The drug discovery laboratory occupies a total of 14, 000 square feet of space on the 9th and 10th floors of the Biomedical Science Tower 3 (BST3) at the Oakland campus. The 11, 600 square feet on the 10th floor is used primarily for exploratory research and assay development, and includes four fully functional tissue culture rooms, five procedure rooms, and two walk-in cold rooms.

Residing on the 9th floor are the high throughput screening (HTS) and High-Content Screening (HCS) facilities and includes a 1, 200 square foot HTS laboratory, 412 square foot HCS laboratory, two fully functional tissue culture rooms, a 201 square foot HTS reagent preparation room, and an additional 414 square foot assay development laboratory.

Within the HTS laboratory is house a Matrical Ministore modular compound storage platform with 3 VLM modules and the capacity for 12, 900 384-well plates or approximately 4.95 million compounds. This system is integrated with LIMS to manage compound storage and retrieval.

Also in the HTS facility is an array of robotic liquid handling systems for bulk delivery of reagents or cells (Titertek Zoom, Titertek MAP-C2, Thermo Scientific Multidrop Combi, Biotek Microflo, and Molecular Devices Aquamax DW4) as well as for compound dilution and transfer to assay or daughter plates (Perkin Elmer Multiprobe, Evolution EP3, and Janus MDT, and the Velocity 11 Bravo). Two BioTek plate washers are available for washing cells in 96- and 384-well plates.

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